yfnB [2019-06-25 08:52:40]

putative [SW|HAD superfamily] phosphatase

locus: BSU07330

pI: 4.63

mw: 27.23 kDa

protein length: 235 aa

gene length: 708 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 804,657 805,364

The protein

Protein family

• [SW|HAD superfamily]
• YjjG family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|50417B2FA0ED2258A13B1E708514F055297599EF|SerB]

Structure

• [PDB|3ED5]

Expression and Regulation

Operons

• genes: [gene|7E180420EB053D9EF7993EF600A169C446F905F9|mtrA]-[gene|2E1E22349750C3EE9B6575EED30A82ED0D5F07F0|yfnB]
  description: [Pubmed|22383849]

• genes: [gene|2E1E22349750C3EE9B6575EED30A82ED0D5F07F0|yfnB]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C327 (yfnB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2325 NBRP B. subtilis, Japan]
• BKE07330 ([gene|2E1E22349750C3EE9B6575EED30A82ED0D5F07F0|yfnB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTGTGTCTCCCTTTC, downstream forward: _UP4_TAATCAGGCTGACGGTATTT
• BKK07330 ([gene|2E1E22349750C3EE9B6575EED30A82ED0D5F07F0|yfnB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTGTGTCTCCCTTTC, downstream forward: _UP4_TAATCAGGCTGACGGTATTT